Ok so this isn't really a problem per-say but i was just curious as to what it really means....
I ran picsrd's insert size metrics on my PE RNA Seq data (mapped to the Ensembl Transcriptome model) and iin the results i get the size of largest insert as 11kb.....
does this mean that a read in a pair has mapped to 1 point and its mate has mapped to another gene somewhere else in the consolidated transcriptome reference, 11kb away, or does it means something else altogether?
I ran picsrd's insert size metrics on my PE RNA Seq data (mapped to the Ensembl Transcriptome model) and iin the results i get the size of largest insert as 11kb.....
does this mean that a read in a pair has mapped to 1 point and its mate has mapped to another gene somewhere else in the consolidated transcriptome reference, 11kb away, or does it means something else altogether?
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