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Old 05-30-2018, 11:49 PM   #10
anineo
Junior Member
 
Location: Trondheim, Norway

Join Date: Mar 2017
Posts: 4
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Hi,
we also had a lot of duplication. We sequenced at 2500x and ended up with a unique depth at around 200x. In our case it may be attributed to low complexity in the sample, as we started with 20 ng cfDNA and possibly even less. However we also eperienced duplication in the tumor DNA samples, which should have been more complex.

How much DNA did you make the libraries from?
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