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zshuhua · 05-10-2013, 07:28 AM

Thanks for your help!
I intend to calculate genes/transcripts expression levels. Do I have to cut the 5' 10 bp?
I'm wondering what causes the patterns of for GGGGG, AAAAA and TCTTC, because I saw those patterns in other tens of RNA-seq data files.

05-10-2013, 07:28 AM	#3
zshuhua Junior Member Location: Ontario Join Date: May 2010 Posts: 4	Thanks for your help! I intend to calculate genes/transcripts expression levels. Do I have to cut the 5' 10 bp? I'm wondering what causes the patterns of for GGGGG, AAAAA and TCTTC, because I saw those patterns in other tens of RNA-seq data files.