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Old 01-07-2015, 07:57 AM   #1
Location: Britain

Join Date: Jan 2015
Posts: 23
Default 16S V4 Indexing PCR - Thoughts on this gel?

Hi all

I'm running a microbiome analysis of fish gut microdlora using Illumina MiSeq 16s pipeline. I'd like to get your thoughts on my indexing PCR gel - some of the bands seem a little faint. Can band strength be an indicator of performance of the samples once pooled and sequenced?

Gel pic attached.

Would really appreciate any insights.

Thank you

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