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Old 01-07-2015, 08:23 AM   #2
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Location: New England

Join Date: Jun 2012
Posts: 200

After the second round of PCR (and cleanup), I quantify with Qubit, convert to nM, and pool equimolar amounts. This works well with 99% of my samples, regardless of how the band on the gel looks. Occasionally, I have a sample pretty much fall out.
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