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Old 01-07-2015, 12:19 PM   #10
Senior Member
Location: New England

Join Date: Jun 2012
Posts: 200

If I was adding 10 ng of DNA to the index PCR, I'd probably do 8 cycles. If your DNA is clean, it should be fine. I've successfully done it with much less. You don't actually need to end up with all that much to run the MiSeq! Try it with a couple of samples and see what you get!
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