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Old 06-18-2010, 01:53 AM   #1
bassu
Junior Member
 
Location: India

Join Date: Jun 2010
Posts: 5
Smile Ngs data analysis

Dear All,

As a part of my curriculum, i am currently planning to do a pipeline like sort on NGS data. Through various reading and other sorts i found that there are some leading tools like bowtie, tophat, Maq, Cufflinks etc.

From NCBI SRA sample of RNASEQ of illumina. but as i am new to these technologies i dont know what are the sequential steps i should follow to get a meaning full result.

what i am trying to analyse is to get the differential expression between two different organ data. How can i identify the control sample(is it needed?), and what are the tools i should use and in which order.

I know this will be a mundane task for some of you, but i am hopefull that you people can help me to sort this out.

Hoping for a positive replay asap
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