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Old 10-05-2018, 09:00 AM   #13
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Location: New England

Join Date: Oct 2018
Posts: 4

Originally Posted by js0402 View Post
Good day Egansbay.

Im also doing the gut microflora using the illumina flatform. I want to ask, after you did your dna extraction does it normally produce a smearing on the gel agarose ?

Hope you can help me on this.
Thank you very much.
If you're still wondering after a month...

Generally no, you should see a tight band with any "normal" 16S amplification. This is because you're amplifying one specific DNA segment (typically the V4 area of the gene coding for 16S ribosomal RNA) and while this segment is variable in sequence (the info you want to know with the analysis), it shouldn't vary in length (what a band on a gel would show).

Hope that helps!
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