View Single Post
Old 03-31-2012, 02:39 PM   #2
Senior Member
Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,317

First, you bioA chip results are hosed in some of the lanes. You can see the fuzzy 10.38kb upper marker has not been correctly identified in several of the lanes. You need to view the .xad file with 2100 Expert software and go to each of those traces, switch to the peak table tab and figure out which peak is your real 10.38 kb band, right-click on it and choose "manually set upper marker". This will give you the correct size of your peaks.

That said, looks to me like the right four lanes have 2 different MW markers in them. Either you samples are contaminated with them, or the pins of your bioA were contaminated with them.

That is my take, anyway.
pmiguel is offline   Reply With Quote