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Old 12-09-2013, 07:08 AM   #5
Michael Love
Senior Member
Location: Boston

Join Date: Jul 2013
Posts: 333

hi John,

The VST helps to stabilize the variance over the mean, insofar as this can be captured by the parametric curve of dispersion over mean. You might also try the rlog transformation, which sometimes performs qualitatively better than the VST (for example, if the size factors vary a lot across samples).
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