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Old 12-18-2012, 06:09 AM   #4
lorendarith
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Just by looking at the parameter... it is because you use just 2% duty cycles, whereas they use 5% for the desired size of 500bp. I am not familiar with what you mean Covaris recommends.

I haven't worked with the E220, only S2 but it's all similar. When I want to change the shearing size I usually just play with the time duration.

Shorter time -> bigger fragments
Longer time -> shorter fragments

I go in steps of 5-10s and that mostly gives me satisfying results. Additionally you can change the duty cycles as well, but this change is more drastic.
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