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Old 12-18-2012, 07:35 AM   #6

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Hm, okay. I've always sheared my HMW DNA with similar parameters, durations of 50-80s, obtained desired insert sizes and never did these time points you mentioned; always worked fine.

Though, you wrote you do this on raw cell lines? That's probably where the difference comes from. Can't help you there, but say to just try out these other parameters and see if it works
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