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Old 12-20-2012, 09:25 AM   #11
Hamid
Senior Member
 
Location: San Diego, CA

Join Date: Sep 2009
Posts: 105
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Hi nfourier,

Simply increasing the processing energy will not work. It will just destroy the epitopes.
Please process your samples according to the Covaris protocol http://covarisinc.com/wp-content/uploads/pn_010145.pdf . You can contact Maria Kotler mariana@danyel.co.il to see how you could obtain a kit. There are also a few other cell preparation protocols that are compatible with Covaris AFA. Ethan Ford who contributes to seqanswers has a protocol that he uses successfully with a few different cell lines. You can search for his posts to locate a link to his protocol.
Meanwhile can you please provide the following information:
1. How do you currently fix your cells?
2. How do you prepare nuclei?
3. for DNA size range analysis do you RNase treat, and proteinase K treat your samples before loading on a gel?

Thank you

hamid
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