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Old 01-06-2013, 03:50 AM   #14
nfourier
Junior Member
 
Location: Israel

Join Date: Dec 2012
Posts: 8
Default Fixation protocol

Thank you Hamid.
I will take your advice and use Ethan's protocol. I prepared the buffers but I have a question regarding cells in suspention. Ethan's protocol has the fixation buffer volumes for adherant cells and the Covaris protocol has volumes for cells in suspention (10^8 or 2-3*10^7). I will have about 10^8 cells in suspention. How much fixation buffer do you recommand to use, 22ml (+1% formaldehyde) like in the Covaris protocol?

Thank you,
Nitsan.
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