SEQanswers - View Single Post - Comparing read depths per gene/exon between samples

shimbalama · 09-09-2014, 10:41 PM

Thanks Brian.

I do all that. What I am trying to do is QC on the negative var calls. So every base in every gene of interest (GOI).

What I am interested in is the mean read depth of every GOI that comes off my machine and whether it is significantly different to the mean depth I have defined as 'gold'. So the question is about statistical analysis only.

09-09-2014, 10:41 PM	#4
shimbalama bioinformatics-help.com Location: Adelaide Join Date: Jul 2014 Posts: 9	Thanks Brian. I do all that. What I am trying to do is QC on the negative var calls. So every base in every gene of interest (GOI). What I am interested in is the mean read depth of every GOI that comes off my machine and whether it is significantly different to the mean depth I have defined as 'gold'. So the question is about statistical analysis only.