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Old 10-20-2015, 04:16 PM   #30
gringer
David Eccles (gringer)
 
Location: Wellington, New Zealand

Join Date: May 2011
Posts: 836
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I've recently discovered that these are transcript sequences, not genomic sequences (despite the initial assurance against that). So the 140,000x coverage and 500x coverage could possibly be for adjacent sequences, and it is [possibly] reasonable to expect target genes with coverage below 500.

Last edited by gringer; 10-20-2015 at 04:19 PM.
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