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Old 02-20-2019, 07:31 PM   #4
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,238
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Paired end sequencing means that library fragments (in this case ITS amplicons) are sequenced from both ends so R1 and R2 of the fragment are in opposite direction. If sequencing cycles are longer than library fragment then R1 nad R2 can be merged (if there is enough overlap). You can ask the sequencing centre to provide at least the workflow and that should answer your question.
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