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Old 08-07-2020, 07:57 AM   #1
geeneticode
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Location: Sevilla, Spain

Join Date: Aug 2020
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Default Is a compositional bias in Sequence Content of initial bases in WES reads normal?

Per Base Sequence Content

Any experienced Whole Exome Sequencing analyst/experimentalist:

the Per Base Sequence Content of the initial couple of bases from the 3' end of my WES reads have some type of compositional bias. Is this some "harmless" procedural or technical bias occurring during sequencing or is it something to be worried about for the downstream analysis?

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