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Old 09-11-2009, 02:15 AM   #1
mchotalia
Junior Member
 
Location: London

Join Date: Jan 2009
Posts: 8
Default Removing an unknown primer sequence from reads.

Hi,

Could anyone help me learn how to remove an unknown primer sequence from the ends of reads. I have these primers attached to the DNA prior to making the Illumina libraries as i have used them for other reactions. This means that when the data is compiled i have a lot of data that is unmappable due to these primers.

Any ideas?

I have to admit...i'm no bioinformatician so could you keep your replies easy for me to understand please.

Thanks in advance
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