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Old 11-17-2014, 09:00 AM   #5
zhaolin
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Location: Ann Arbor

Join Date: Nov 2014
Posts: 4
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Quote:
Originally Posted by obischof View Post
We did; always smear no matter how long or short you incubate.
What is the size of your library smear? I have tried to lower the transposase amount in the reaction and found the size of PCR product changes. But it still a piece of smear. How much of PCR product did you check on gel? Is it too low amount in the protocol?
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