View Single Post
Old 12-11-2018, 07:34 AM   #3
Location: Muenster

Join Date: Apr 2016
Posts: 14

The two bioanalyzer pictures from OP actually look OK to me, clear nucleosomal periodicity. Note that the peak at 180bp is not the mono-nucleosomal but the nucleosome-free peak. From the 180bp, you have to subtract about 100bp for adapter content, leaving you with 80bp inserts which is from nucleosome-free DNA. Fragments of about 200bp are mono-nucleosomal ones.

@Grg91 Can you post a picture of your libraries, so that we can have a look.
atpoint is offline   Reply With Quote