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Old 12-12-2018, 02:26 AM   #7
atpoint
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Location: Muenster

Join Date: Apr 2016
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Yes, this is most likely overtagmentated. It is also too much heat. ATAC-seq works at 37C for 30' with larger genomes like human and mouse. Worm genomes are much smaller, and therefore you might need to optimize the time and enzyme amount for it. Still, do not go for 55C. The idea is to leave the chromatin at a native state, and 55C is not compatible with that plus the transposase is too active. Maybe take a published protocol for Drosophila to get inspiration. I guess the worm genome and Drosophila genomes are more compatible in size than worm and human/mouse.
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