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Old 08-07-2019, 08:17 PM   #10
firsal
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Location: Sweden

Join Date: Oct 2014
Posts: 10
Default Bioanalyser data of ATAC-seq library

Hi,

I have run ATAC-seq library preparation for the first time. I need advice about my Bioanalyser result. It seems the mono-nucleosomal ones are not in high concentration compared to multiple nucleosomes. I used 50,000 human cell line. I also followed the OMNI-ATAC-seq protocol and did amplification for 8 cycles.

What optimizations I should do to get the optimal result. The DNA size is based on second and 60 sec relates to 200 bp.

I really appreciate your feedback and comments.
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