View Single Post
Old 03-09-2018, 10:17 AM   #1
Junior Member
Location: usa

Join Date: Mar 2018
Posts: 1
Default primer-dimer or adaptor-dimer

Hi, all,
I just prepared couple of libraries with the NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina. After run them on a Qiaxcel system, I saw a visible ~35bp peak.(15bp and 3000bp are alignment markers) Could it be primer-dimer or adaptor-dimer? Should I perform another round of beads clean-up? Or I can just send them for sequencing?
Attached Images
File Type: jpeg 03-08-2018 264.jpeg (117.0 KB, 70 views)
woodyzc is offline   Reply With Quote