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Old 09-24-2018, 05:33 AM   #5
Location: Hanover, Germany

Join Date: Sep 2018
Posts: 15

Ah, low diversity between the clusters in each sequencing cycle, I understand how that would be problematic.
Yes, it is a V3 run.
And I see how a overclustering of smaller inserts would lead to sequencing into the opposite adapter with low nucleotide diversity. As soon as I get a PC and access to the institutes server I will look into the linked method of determining the true insert length.
As I see the dropoff after around 100 bases, I should also see that as the true insert length, right? And that should be pretty salient in the data?

As my supervisor will only return next week and my PC is still not set up, I won't be able to look up the phiX controls but I remember him saying that they looked okay.
Thank you for your help so far, I'll will post any developments.
JasperGeh is offline   Reply With Quote