View Single Post
Old 09-02-2013, 07:49 PM   #1
WaitingNail
Junior Member
 
Location: Taiwan

Join Date: Jun 2013
Posts: 3
Default Somatic mutation analysis

Dear all

I have a project to analysis somatic mutation from clinical sample, maybe serum.

I will choose some gene and design PCR primer to amplify target exon region.

These amplicon will sequenced over 500X by using Illuminar Miseq 150PE.

My question is, how to analysis or define "somatic mutation".

Because the sequence depth is really high, I think the sequence data will has some sequence error to interfere somatic mutation calling, how can I filter out this possible bias? Reads trimming using QV 30 instead of QV20?

Thanks for any help,

WT
WaitingNail is offline   Reply With Quote