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Old 02-06-2009, 05:02 PM   #20
danielfortin86
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Location: California

Join Date: Aug 2008
Posts: 5
Default Adapter sequence

i know that the overhanging T is used to ligate with the added A from the DNA library, but thy is there a one base pair overhang on the other side? If you see how the sequence anneal there is an overhang on both ends. Both adapter sequences are 33 bases. If you only wanted the T overhang, why wouldn't you have 32 bases on one strand and 33 on the strand with the T overhang?
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