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Old 09-24-2021, 05:14 PM   #2
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Join Date: Dec 2010
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You will not need a library prep kit. You can add all the required Illumina sequences by PCR (in one round or two rounds). This might give you an idea: https://dnatech.genomecenter.ucdavis...umina-systems/
Your libraries will have a very low complexity by default. Thus, you will need to sequence your amplicon pool together with PhiX or another complex library.
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