Hi,
do you have experience with good results of illumina libraries from Agilent, but qPCR says that there are not anything? I prepared library (DNA, TruSeq), measured it with qPCR and Agilent, everything was OK and friezed it. Before sequencing (1week) I did Agilent and qPCR again and according to qPCR there were not anything. Is is possible that DNA de-gradated so fast, or is it some contamination that damage DNA or something else? I did a new library and new library with new kit and qPCR KO, agilent OK.
Thanks for advice.
do you have experience with good results of illumina libraries from Agilent, but qPCR says that there are not anything? I prepared library (DNA, TruSeq), measured it with qPCR and Agilent, everything was OK and friezed it. Before sequencing (1week) I did Agilent and qPCR again and according to qPCR there were not anything. Is is possible that DNA de-gradated so fast, or is it some contamination that damage DNA or something else? I did a new library and new library with new kit and qPCR KO, agilent OK.
Thanks for advice.
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