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Old 03-13-2019, 06:24 AM   #4
Location: us

Join Date: Aug 2015
Posts: 41

Thank you very much!
I simplified the questions!

Originally Posted by Gopo View Post
The "fragment" of 1000bp in your example would be flanked by sequencing adapters in traditional Illumina sequencing, but really, you would call what you are calling the "fragment" as the insert sequence and the insert plus the sequencing adapters would be the entire fragment. I would agree with your understanding of how the rest of the 850bp are sequenced except that you rarely have a fragment (insert + sequencing adapters) size distribution with only fragments of 1000bp (rather it follows some distribution) consisting of smaller and larger fragments. Note that fragments resulting from PCR and then Illumina library prep can follow a tight length distribution.
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