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Old 01-06-2011, 08:26 AM   #1
obischof
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Location: france

Join Date: Jan 2011
Posts: 11
Unhappy Agilent Bioanalyzer 2100 detects no DNA

Dear All,

I have run into a huge problem with our seq platform. The problem is as follows. We have prepared chromatin using Mn digestion after PFA XL. We verified the appearance of primarily mono-nucleosomes in an agarose gel. We then went ahead to chip using different abs vs histone mods. The chipped material we again analysed on a gel and we saw nice bands in the vicinity of 100-200. This material we had analysed by the Agilent Bioanalyzer but zilch. So, we ignored this in the meantime because the gel was fine. Next, we prepared the library and ran it on the Bioanalyzer. Now, we saw a nice band around 200bp-ish, which we thought was fabulous (see photo). So, we made a test run on the Illumina Sequencer and to our suprise zilch. Now, what in heavens name is happening here. If someone could help me out.

Thank you
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File Type: pdf LIB-OLIVER-171210_DNA_1000_2010-12-17_001-1.pdf (487.3 KB, 136 views)
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