Hey all,
Anyone has tried using the MiSeq v3 chemistry yet? We just bought a kit and im planning a run next week, and i wanted to see what would you guys recommend concerning lib size selection/and loading concentration for an optimal cluster density.
Illumina announced that the optimal cluster density with the new kit will be around 1200-1500k/mm2. Using 4nM starting concetration, would that still be enough to get this much clusters? With my previous experience, I usually load 12pM and get a cluster den of 860k/mm2. You reckon if i load 20pM with the new chemistry that would be enough?
Best,
Elias
Anyone has tried using the MiSeq v3 chemistry yet? We just bought a kit and im planning a run next week, and i wanted to see what would you guys recommend concerning lib size selection/and loading concentration for an optimal cluster density.
Illumina announced that the optimal cluster density with the new kit will be around 1200-1500k/mm2. Using 4nM starting concetration, would that still be enough to get this much clusters? With my previous experience, I usually load 12pM and get a cluster den of 860k/mm2. You reckon if i load 20pM with the new chemistry that would be enough?
Best,
Elias
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