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Old 06-22-2011, 11:55 AM   #6
kwatts59
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Location: nevada

Join Date: Apr 2011
Posts: 46
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I believe the read quality is the column after the sequence. For example, the quality for the read "GCTGGGTCTGAACTCT" is "IIIIIIIIIIBGGGGI" where
"B" represents a Phred quality score of 33 (Assuming Sanger scoring)
"G" represents a Phred quality score of 38
"I" represents a Phred quality score of 40
To convert the letter to the Phred, just take the ASCII representation and subtract 33.
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