De novo genome assembly: beginner
Hi folks!!
I too am completely new to the NGS. and I am struggling with a question:
What decides the 'amount of data' needed for a de novo genome assembly for a particular organism??????? What decides the insert size during the library construction???
How to decide important parameters such as coverage, size, accuracy, and sensitivity; library type (fragment or mate paired?); and read length.
Any one can please help me!!
many thanks in advance!!
shal
Hi folks!!
I too am completely new to the NGS. and I am struggling with a question:
What decides the 'amount of data' needed for a de novo genome assembly for a particular organism??????? What decides the insert size during the library construction???
How to decide important parameters such as coverage, size, accuracy, and sensitivity; library type (fragment or mate paired?); and read length.
Any one can please help me!!
many thanks in advance!!
shal
Comment