I have been obtained the RNA-seq data of a sample on illumina. The % base calls plot of this lane is below, Is it reasonable? Or what is the problem with it? Why?
And,do you think it is important to optimize adapter oligo concentrations during ligation to the cDNA ends? Why?
And,do you think it is important to optimize adapter oligo concentrations during ligation to the cDNA ends? Why?
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