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Old 12-04-2008, 07:30 AM   #14
berthasalco
Junior Member
 
Location: Germany

Join Date: Dec 2008
Posts: 1
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Hello there,

first at all, i want to thank you to all to coperate to give us the sequence of the GEX 1 and 2 adapters and primers for the tag profilling with DpnII , but

does anybody know at which concentration are they used??? The Illumina protocol does not give concentrations. According to the Invitrogen LongSAGE protocol the double stranded adapters have a concentration of 40 ng/ul and 1.5 ul are used for one ligation reaction

thanks a lot,
BS
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