I am curious about why the Ampure XP protocol calls for 70% Ethanol, while their SPRI Select (supposedly the same as Ampure XP with extra QC) calls for 85% Ethanol.
Has anyone used 70% with the SPRI Select product from Beckman?
Has anyone done an Ethanol concentration titration experiment to see if there is any effect on size selection?
I've read that lower than 70% Ethanol impacts yield, while higher than 70% Ethanol concentration results in inefficient washing away of smaller molecules. What is the mechanism? Anypne have any data?
Reference from Nature Protocols: http://www.gmo-qpcr-analysis.com/tan...ocols-2010.pdf
Thanks for your advice!
Has anyone used 70% with the SPRI Select product from Beckman?
Has anyone done an Ethanol concentration titration experiment to see if there is any effect on size selection?
I've read that lower than 70% Ethanol impacts yield, while higher than 70% Ethanol concentration results in inefficient washing away of smaller molecules. What is the mechanism? Anypne have any data?
Reference from Nature Protocols: http://www.gmo-qpcr-analysis.com/tan...ocols-2010.pdf
Thanks for your advice!
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