Hi all, I've just finished preparing 72 samples for sequencing, these have prepared using index primers and so I want to pool them together before running on the sequencer. I've been told I need 10ul of a 10nM pool for each run but I'm not sure how to convert my individual concentrations (currently in ng/ul) into nano Moles? Also, I've quantified my final libraries using pico green, do people think this is an accurate way to quantify them?
Thanks for any help!
Thanks for any help!
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