SEQanswers - View Single Post - Inefficient ATAC-seq library prep amplification

lapensee · 03-20-2019, 10:16 AM

FWIW I got this working. I adopted the OMNI-ATAC protocol and get great library amplification from 5k cells using only 2% the amount of Tn5 required for 50k cells. Adding a protease inhibitor during transposition was crucial for my library prep.

03-20-2019, 10:16 AM	#5
lapensee Junior Member Location: Michigan Join Date: Oct 2018 Posts: 6	FWIW I got this working. I adopted the OMNI-ATAC protocol and get great library amplification from 5k cells using only 2% the amount of Tn5 required for 50k cells. Adding a protease inhibitor during transposition was crucial for my library prep.