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Old 08-18-2019, 10:30 PM   #1
Carcharodon
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Location: Honolulu, HI

Join Date: Jul 2015
Posts: 40
Default Removal of carry-over EtOH after bead purification of DNA

I recently purified some DNA (double-digest of genomic DNA) with SPRI beads, and I don't believe I let them dry long enough to get rid of excess EtOH used during the rinse step before eluting the DNA with HPLC water.

I read somewhere that placing the DNA solution on a SpeedVac for ~ 2 min should get rid of the ethanol, but I'm skeptical. Would that actually work, especially with such dilute EtOH?

Thanks in advance!
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