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Old 09-27-2019, 01:42 PM   #19
kumard
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Location: Midwest USA

Join Date: Jun 2019
Posts: 6
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Quote:
Originally Posted by Papaveraceae View Post
Has anyone used RNAzol, from Molecular Research Center, they make the claim that DNAse is not required after extraction. See the link: http://www.mrcgene.com/rnazol.htm

Dave
Its a very old thread, but let me add that I am using RNAZol RT and I see DNA contamination even after following their protocol. I am struggling with this problem. I talked to their tech support and they informed me to do DNAse I treatment. The advantages that RNAZol RT have is that you can use water for the phase separation and the smallRNA fraction profile is better (on small RNA sizing on Agilent bioanalyzer) than Trizol (with RNA of RIN 9). I hope it will help someone.
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