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Old 10-18-2019, 11:25 AM   #6
LVAndrews
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Location: Flagstaff, AZ

Join Date: Sep 2012
Posts: 55
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Ran the RNA kit (DNF-471) with an injection of 5.5kV for 10sec, and everything worked well. These were samples that had been run previously, and I only loaded 1uL per sample while also bringing the total volume to 50uL with 0.1% Tween-20. Had to monkey with the ladder a bit to make the software happy, but this may be due to the increased volume. I have modified the injection again to 6kV for 15sec to further ensure adequate injection.
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