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Old 10-01-2012, 08:58 AM   #12
pmiguel
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,315
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Quote:
Originally Posted by Johan de Bondt View Post
If someone would be interested, we have developed an IP protected magnetic bead with active DNase on the surface. Perhaps an elegant manner of removing the DNA without loosing sample or diluting it! If anyone would be interested in this feel free to send me a mail.
Problem would be the DNA would degrade, but be left in solution with the RNA. So you end up having to do a size fractionation anyway to remove it.

I actually think the Zymo DNA-free RNA purification is the way to go for this. Your RNA prep is done and fairly clean. Hit it with a DNAse followed by a column clean-up to both remove the degraded DNA as well as the DNAse. As a bonus you also remove any other residual proteins or contaminants still remaining in your prep.

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Phillip
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