Hi all,
I'm a beginner in sequence assembly and have a question about using the assembler, PHRAP.
I'm trying to use PHRAP to assemble 454 contigs (generated by MIRA) and Sanger reads. However, PHRAP requires its input to follow the St. Louis naming convention, and my 454 contigs clearly do not conform to this system since they are outputs from MIRA; also, they don't contain any info that allows me to convert their names to St. Louis names.
Has anyone encountered similar problems before? And how did one resolve naming issue like this?
Thanks.
I'm a beginner in sequence assembly and have a question about using the assembler, PHRAP.
I'm trying to use PHRAP to assemble 454 contigs (generated by MIRA) and Sanger reads. However, PHRAP requires its input to follow the St. Louis naming convention, and my 454 contigs clearly do not conform to this system since they are outputs from MIRA; also, they don't contain any info that allows me to convert their names to St. Louis names.
Has anyone encountered similar problems before? And how did one resolve naming issue like this?
Thanks.
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