Dear all,
I sheared lymphoblastoid cell line (LCL) samples and whole blood samples by covaris S2. Then, I ran bioanalyzer to check their length.
Interestingly, unlike whole blood samples that showed typical graph after covaris shearing, sheared LCL samples looked different
First, a bit wider than whole blood samples
Second, low fluorescent unit -> I measured conc' again with nanodrop but same as whole blood.
Third, their graph started from little bit higher than baseline.
I assumed that fragmentation of episome is a reason why a bunch of small fragments are. But I cannot understand low concentration and wider distribution.
I sheared lymphoblastoid cell line (LCL) samples and whole blood samples by covaris S2. Then, I ran bioanalyzer to check their length.
Interestingly, unlike whole blood samples that showed typical graph after covaris shearing, sheared LCL samples looked different
First, a bit wider than whole blood samples
Second, low fluorescent unit -> I measured conc' again with nanodrop but same as whole blood.
Third, their graph started from little bit higher than baseline.
I assumed that fragmentation of episome is a reason why a bunch of small fragments are. But I cannot understand low concentration and wider distribution.
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