SEQanswers - View Single Post - Questions for barcode file splitting, forward/reverse data sorting

rzeng · 08-21-2013, 01:33 PM

GenoMax,

I have splitted 400,000,000 tag reads and grouped them into 6 separate files using OUTER 6 different barcode sequences. I want to confirm with you that the next step is to use my own script or from Qiime to split files in R1 and R2 using EACH of 6 separate files, right? since R1 and R2 do not have the barcode tags (or barcode sequence) but similar ID headline (highlighted as RED as follows).

R1 file
@IPAR1:2:1:4029:1196:1#0/1

Splitted barcode file
@IPAR1:2:1:4029:1196:1#0/2

R2 file
@IPAR1:2:1:4029:1196:1#0/3

Can script of Qiime help me to split R1 and R2 just by using these RED hightlight headline?

08-21-2013, 01:33 PM	#9
rzeng Member Location: houston Join Date: Aug 2013 Posts: 19	GenoMax, I have splitted 400,000,000 tag reads and grouped them into 6 separate files using OUTER 6 different barcode sequences. I want to confirm with you that the next step is to use my own script or from Qiime to split files in R1 and R2 using EACH of 6 separate files, right? since R1 and R2 do not have the barcode tags (or barcode sequence) but similar ID headline (highlighted as RED as follows). R1 file @IPAR1:2:1:4029:1196:1#0/1 Splitted barcode file @IPAR1:2:1:4029:1196:1#0/2 R2 file @IPAR1:2:1:4029:1196:1#0/3 Can script of Qiime help me to split R1 and R2 just by using these RED hightlight headline? Last edited by rzeng; 08-21-2013 at 01:37 PM.