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Old 08-21-2013, 01:33 PM   #9
Location: houston

Join Date: Aug 2013
Posts: 19


I have splitted 400,000,000 tag reads and grouped them into 6 separate files using OUTER 6 different barcode sequences. I want to confirm with you that the next step is to use my own script or from Qiime to split files in R1 and R2 using EACH of 6 separate files, right? since R1 and R2 do not have the barcode tags (or barcode sequence) but similar ID headline (highlighted as RED as follows).

R1 file

Splitted barcode file

R2 file

Can script of Qiime help me to split R1 and R2 just by using these RED hightlight headline?

Last edited by rzeng; 08-21-2013 at 01:37 PM.
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