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Old 09-24-2013, 09:25 AM   #3
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Location: Auburn, AL

Join Date: May 2012
Posts: 8

Originally Posted by dpryan View Post
This will depend upon how you do your mapping and then the expression quantitation.
I used RNA-seq analysis in CLC genomic workbench.
Set parameters is as below
Minimum length fraction: 0.9
Minimum similarity fraction: 0.8
Maximum number of hits for a read: 10
What is the right way to do the mapping and quantitation for a small subset reference? I really appreciate if you can provide a reference for this. Thank you very much!
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