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Old 11-13-2013, 01:47 PM   #1
FLYINGDOLPHIN
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Location: washington dc

Join Date: Apr 2013
Posts: 11
Default extra sequence following transcript in RNA-seq ?

Hi,

I did a dual-seq with pathogen and host. I found more nucleotides after the total mRNA transcript(as annotation) from the pathogen. For each sequence that mapped to the pathogen transcript in the fastq file, the strings of nuceotides following the end of the transcript, however are very different. This role out the possibility that the annotation sequence is not intact.

Does anyone know whether this happens often? And why this would happen?

Thanks a lot!

Best,
F
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