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Old 04-09-2014, 10:26 PM   #1
Location: USA

Join Date: Feb 2014
Posts: 40
Default Normalizing by Library Amount

I got my Seq data back and processed with bow tie and htseq and I have my mapped gene counts... in my library I see that this is how much DNA was used for sequencing by the staff

615 ng ---- Sample 1
423 ng ---- Sample 2
426 ng ----- Sample 3

Can I divide the total mapped reads by these numbers to get a kind of weighted normalization?

What would be the danger of doing that?
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