I got my Seq data back and processed with bow tie and htseq and I have my mapped gene counts... in my library I see that this is how much DNA was used for sequencing by the staff
615 ng ---- Sample 1
423 ng ---- Sample 2
426 ng ----- Sample 3
Can I divide the total mapped reads by these numbers to get a kind of weighted normalization?
What would be the danger of doing that?
615 ng ---- Sample 1
423 ng ---- Sample 2
426 ng ----- Sample 3
Can I divide the total mapped reads by these numbers to get a kind of weighted normalization?
What would be the danger of doing that?
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